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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Lamin B1 Monoclonal antibody (66095-1-Ig)

    WB analysis using 66095-1-Ig (same clone as 66095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66095-1-Ig (Lamin B1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of multi-cells/tissue using Lamin B1 Monoclonal antibody (66095-1-Ig)

    WB analysis of multi-cells/tissue using 66095-1-Ig (same clone as 66095-1-PBS)

    Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Lamin B1 Monoclonal antibody (66095-1-Ig)

    WB analysis of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    WB result of Lamin B1 antibody (66095-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Sample test of MP51325-1

    Sample test of MP51325-1

    Sample test of MP51325-1, Lamin B1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66095-1-PBS. Detection antibody: 66095-2-PBS.

  • Immunoprecipitation (IP) experiment of HeLa cells using Lamin B1 Monoclonal antibody (66095-1-Ig)

    IP experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    IP result of anti-Lamin B1 (IP:66095-1-Ig, 4ug; Detection:66095-1-Ig 1:20000) with HeLa cells lysate 3560ug. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Lamin B1 Monoclonal antibody (66095-1-Ig)

    IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Lamin B1 Monoclonal antibody (66095-1-Ig)

    IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Lamin B1 Monoclonal antibody (66095-1-Ig)

    IHC staining of human breast cancer using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using Lamin B1 Monoclonal antibody (66095-1-Ig)

    IF Staining of mouse eye using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Lamin B1 antibody (66095-1-Ig, Clone: 3C10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin B1 Monoclonal antibody (66095-1-Ig)

    IF Staining of HepG2 using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Lamin B1 Monoclonal antibody (66095-1-Ig)

    IF Staining of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Lamin B1 Monoclonal antibody (66095-1-Ig)

    FC experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (66095-1-Ig, Clone:3C10G12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51325-1

    Cytometric bead array standard curve of MP51325-1

    Cytometric bead array standard curve of MP51325-1, Lamin B1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66095-1-PBS. Detection antibody: 66095-2-PBS. Standard:Ag20522. Range: 0.391-200 ng/mL.

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  • Featured Product
  • KD/KO Validated

Lamin B1 Monoclonal antibody, PBS Only (Capture)

Lamin B1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 66095-1-PBS
Clone No.3C10G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

LMNB1, 3C10G12, laminB1, Lamin-B1, LMN2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66095-1-PBS targets Lamin B1 as part of a matched antibody pair:

MP51325-1: 66095-1-PBS capture and 66095-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Lamin B1 fusion protein Ag20522 相同性解析による交差性が予測される生物種
Full Name lamin B1
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC012295
Gene Symbol Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_11232208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20700
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.