Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 20878-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 20878-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20878-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 20878-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 20878-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20878-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 20878-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 20878-1-AP
IP result of anti-LZTS1 (IP:20878-1-AP, 3ug; Detection:20878-1-AP 1:500) with mouse brain tissue lysate 7500ug.
IHC staining of human skin cancer using 20878-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 20878-1-AP (LZTS1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 20878-1-AP
Immunofluorescent analysis of HepG2 cells, using LZTS1 antibody 20878-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

LZTS1 Polyclonal antibody

LZTS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20878-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Fez1, F37/esophageal cancer-related gene-coding leucine-zipper motif, F37

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, human testis tissue, mouse ovary tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

20878-1-AP targets LZTS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LZTS1 fusion protein Ag14977 相同性解析による交差性が予測される生物種
Full Name	leucine zipper, putative tumor suppressor 1
Calculated molecular weight	596 aa, 67 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC075007
Gene Symbol	LZTS1
Gene ID (NCBI)	11178
RRID	AB_10693683
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y250
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for LZTS1 antibody 20878-1-AP	Download protocol
IHC protocol for LZTS1 antibody 20878-1-AP	Download protocol
IF protocol for LZTS1 antibody 20878-1-AP	Download protocol
IP protocol for LZTS1 antibody 20878-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development. Authors - T Kawaue KO Validated View Article
human	WB	Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis Authors - Tianwei Chen View Article
human	WB,IHC	Int Dent J Screening Differentially Expressed Proteins in Areca Nut-Related Oral Squamous Cell Carcinoma Using Tandem Mass Tag Proteomics Authors - Xiaoqun Mai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20878-1-AP

WB analysis of human testis using 20878-1-AP

WB analysis of Jurkat using 20878-1-AP

WB analysis of PC-3 using 20878-1-AP

WB analysis of mouse thymus using 20878-1-AP

WB analysis of HEK-293 using 20878-1-AP

WB analysis of mouse ovary using 20878-1-AP

IP experiment of mouse brain using 20878-1-AP

IHC staining of human skin cancer using 20878-1-AP

IF Staining of HepG2 using 20878-1-AP