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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LYVE1 Polyclonal antibody (34236-1-AP)

    WB analysis using 34236-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 34236-1-AP (LYVE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using LYVE1 Polyclonal antibody (34236-1-AP)

    IHC staining of mouse colon using 34236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 34236-1-AP (LyVE-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using LYVE1 Polyclonal antibody (34236-1-AP)

    IHC staining of mouse colon using 34236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 34236-1-AP (LyVE-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LYVE1 Polyclonal antibody (34236-1-AP)

    IHC staining of mouse liver using 34236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 34236-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LYVE1 Polyclonal antibody (34236-1-AP)

    IHC staining of mouse liver using 34236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 34236-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using LYVE1 Polyclonal antibody (34236-1-AP)

    IF Staining of rat liver using 34236-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using LYVE1 antibody (34236-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LYVE1 Polyclonal antibody

LYVE1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 34236-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Cell surface retention sequence-binding protein 1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Crsbp1, CRSBP-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inbEnd.3 cells, mouse lung tissue
Positive IHC detected inmouse liver tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

34236-1-AP targets LYVE1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg6367

Product name: Recombinant mouse LyVE-1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-234 aa of NM_053247.4

Sequence: ADLVQDLSISTCRIMGVALVGRNKNPQMNFTEANEACKMLGLTLASRDQVESAQKSGFETCSYGWVGEQFSVIPRIFSNPRCGKNGKGVLIWNAPSSQKFKAYCHNSSDTWVNSCIPEIVTTFYPVLDTQTPATEFSVSSSAYLASSPDSTTPVSATTRAPPLTSMARKTKKICITEVYTEPITMATETEAFVASGAAFKNEAAGFGGVPT

相同性解析による交差性が予測される生物種
Full Name lymphatic vessel endothelial hyaluronan receptor 1
Calculated molecular weight35 kDa
Observed molecular weight 55 kDa, 60-70 kDa
GenBank accession numberNM_053247.4
Gene Symbol Lyve1
Gene ID (NCBI) 114332
RRIDAB_3743178
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8BHC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

Protocols

Product Specific Protocols
IF protocol for LYVE1 antibody 34236-1-APDownload protocol
IHC protocol for LYVE1 antibody 34236-1-APDownload protocol
WB protocol for LYVE1 antibody 34236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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