Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, bEnd.3 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue |
| Positive IHC detected in | rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
28321-1-AP targets LYVE1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28131 Product name: Recombinant human LYVE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 192-322 aa of BC026231 Sequence: SIPRRKKLICVTEVFMETSTMSTETEPFVENKAAFKNEAAGFGGVPTALLVLALLFFGAAAGLGFCYVKRYVKAFPFTNKNQQKEMIETKVVKEEKANDSNPNEESKKTDKNPEESKSPSKTTVRCLEAEV 相同性解析による交差性が予測される生物種 |
| Full Name | lymphatic vessel endothelial hyaluronan receptor 1 |
| Calculated molecular weight | 322 aa, 35 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC026231 |
| Gene Symbol | LYVE1 |
| Gene ID (NCBI) | 10894 |
| RRID | AB_3086043 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Y7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LYVE1 antibody 28321-1-AP | Download protocol |
| IHC protocol for LYVE1 antibody 28321-1-AP | Download protocol |
| WB protocol for LYVE1 antibody 28321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Adv Res Aquaporin 4 and its isoforms regulation ameliorate AQP4 Mis-localization-induced glymphatic dysfunction in ischemic stroke | ||
Exp Eye Res Distinct Activation of M1 and M2 Macrophages in the Primary Pterygium Lymphangiogenesis |