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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LYVE-1 Polyclonal antibody (32649-1-AP)

    WB analysis using 32649-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32649-1-AP (LYVE-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LYVE-1 Polyclonal antibody (32649-1-AP)

    WB analysis using 32649-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32649-1-AP (LYVE-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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LYVE-1 Polyclonal antibody

LYVE-1 Polyclonal Antibody for WB, ELISA
Cat No. 32649-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LYVE1, HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, bEnd.3 cells, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32649-1-AP targets LYVE-1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYVE-1 fusion protein Eg1615 相同性解析による交差性が予測される生物種
Full Name lymphatic vessel endothelial hyaluronan receptor 1
Calculated molecular weight35kDa
Observed molecular weight55 kDa
GenBank accession numberNM_006691.4
Gene Symbol LYVE1
Gene ID (NCBI) 10894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Y7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

Protocols

Product Specific Protocols
WB protocol for LYVE-1 antibody 32649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols