Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
24313-1-AP targets LYSMD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19430 Product name: Recombinant human LYSMD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC136741 Sequence: MAGRHQNRSFPLPGVQSSGQVHAFGNCSDSDILEEDAEVYELRSRGKEKVRRSTSRDRLDDIIVLTKDIQEGDTLNAIALQYCCTVADIKRVNNLISDQDFFALRSIKIPVKKFSSLTETLCPPKGRQTSRHSSVQYSSEQQ 相同性解析による交差性が予測される生物種 |
| Full Name | LysM, putative peptidoglycan-binding, domain containing 3 |
| Calculated molecular weight | 306 aa, 35 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC136741 |
| Gene Symbol | LYSMD3 |
| Gene ID (NCBI) | 116068 |
| RRID | AB_2879490 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z3D4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LYSMD3 also termed as LysM and putative peptidoglycan binding domain containing protein 3 is a 306 amino acid single-pass membrane protein that contains one LysM repeat. LysM domain encoding genes may play a role in brain and in nervous system development and functioning. LYSMD3, shows a high level of central nervous expression during embryogenesis and is also, therefore, a good candidate gene for other central nervous system (CNS) symptoms, such as psychomotor retardation, brain anomalies and muscular hypotonia of the 5q14.3 microdeletion syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LYSMD3 antibody 24313-1-AP | Download protocol |
| WB protocol for LYSMD3 antibody 24313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens. | ||
Mol Biol Cell Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response. | ||
