Validation Data Gallery
Tested Applications
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31548-1-AP targets LYRM5 in IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35889 Product name: Recombinant human LYRM5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-88 aa of BC071769 Sequence: MANSLRGEVLKLYKNLLYLGRDYPKGADYFKKRLKNIFLKNKDVKNPEKIKELIAQGEFVMKELEALYFLRKYRAMKQRYYSDTNKTN 相同性解析による交差性が予測される生物種 |
| Full Name | LYR motif containing 5 |
| Observed molecular weight | 11 kDa |
| GenBank accession number | BC071769 |
| Gene Symbol | LYRM5 |
| Gene ID (NCBI) | 144363 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6IPR1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LYRM5, also known as ETFRF1, belongs to the Complex1_LYR-like Superfamily. LYRM5 is a mitochondrial protein and plays key roles in acetate metabolism, and in essential Fe-S cluster biogenesis. In vitro, LYRM5 was found to inhibit ETF by promoting the removal of flavin from the ETF holoenzyme, thus potentially regulating the rate of β-oxidation (PMID: 35402183, PMID: 36266680). The calculated molecular weight of LYRM5 is 11 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for LYRM5 antibody 31548-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |