Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
16146-1-AP targets LYPLAL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9271 Product name: Recombinant human LYPLAL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC016711 Sequence: MAAASGSVLQRCIVSPAGRHSASLIFLHGSGDSGQGLRMWIKQVLNQDLTFQHIKIIYPTAPPRSYTPMKGGISNVWFDRFKITNDCPEHLESIDVMCQVLTDLIDEEVKSGIKKNRILIGGFSMGGCMAMHLAYRNHQDVAGVFALSSFLNKASAVYQALQKSNGVLPELFQCHGTADELVLHSWAEETNSMLKSLGVTTKFHSFPNVYHELSKTELDILKLWILTKLPGEMEKQK 相同性解析による交差性が予測される生物種 |
| Full Name | lysophospholipase-like 1 |
| Calculated molecular weight | 237 aa, 26 kDa |
| Observed molecular weight | 26-30 kDa |
| GenBank accession number | BC016711 |
| Gene Symbol | LYPLAL1 |
| Gene ID (NCBI) | 127018 |
| RRID | AB_2138521 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VWZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lysophospholipase-like 1 protein, encoded by LYPLAL1, a 26 kDa cytosol protein which belongs to a subclass of lysophospholipase family. Human genome-wide association studies link variants of the gene encoding this enzyme to fat distribution, waist-to-hip ratio and non-alcoholic fatty liver disease. LYPLAL1 reported to be up-regulated in subcutaneous adipose tissue of obese individuals.(PMID: 25958046, PMID: 27391855, PMID: 21674055)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LYPLAL1 antibody 16146-1-AP | Download protocol |
| WB protocol for LYPLAL1 antibody 16146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Discovery of small-molecule enzyme activators by activity-based protein profiling. | ||
Mol Cell Targeting LYPLAL1-mediated cGAS depalmitoylation enhances the response to anti-tumor immunotherapy | ||
J Mol Endocrinol Knockout of murine Lyplal1 confers sex-specific protection against diet-induced obesity |
