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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LYPD3 Recombinant antibody (83345-1-RR)

    WB analysis using 83345-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83345-1-RR (LYPD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83345-1-RR

    Affinity and Kinetic Characterization of 83345-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83345-1 -RR against Human LYPD3 were performed. The affinity constant is 3.04 nM.

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LYPD3 Recombinant antibody

LYPD3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83345-1-RR
Clone No.240347C5
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MIG C4, LY6/PLAUR domain containing 3, C4.4A, 240347C5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HaCaT cells, SK-BR-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

83345-1-RR targets LYPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LYPD3 fusion protein Ag34961 相同性解析による交差性が予測される生物種
Full Name LY6/PLAUR domain containing 3
Calculated molecular weight36 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberNM_014400
Gene Symbol LYPD3
Gene ID (NCBI) 27076
RRIDAB_3671004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95274
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LYPD3 (Ly6/PLAUR domain-containing protein 3), also known as C4.4A , is a membrane protein anchored to the cell surface via a glycosylphosphatidylinositol (GPI) moiety (PMID: 25302166; 15012588). It is a tumorigenic and high-glycosylated protein that has been proven to be linked with the carcinogenic effects in different solid tumors, such as lung cancer and breast cancer (PMID: 32040344; 29371917; 31644911). It has been shown that the elevated expression of LYPD3 is associated with lung adenocarcinoma carcinogenesis and poor prognosis (PMID: 25302166; 32040344).

Protocols

Product Specific Protocols
WB protocol for LYPD3 antibody 83345-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cell Mol Med

Unveiling Varied Cell Death Patterns in Lung Adenocarcinoma Prognosis and Immunotherapy Based on Single-Cell Analysis and Machine Learning

Authors - Zipei Song
View Article