Validation Data Gallery
Filter:
Tested Applications
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15946-1-AP targets LYL1 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LYL1 fusion protein Ag8528 相同性解析による交差性が予測される生物種 |
| Full Name | lymphoblastic leukemia derived sequence 1 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC002796 |
| Gene Symbol | LYL1 |
| Gene ID (NCBI) | 4066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12980 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lymphoblastic leukemia1 (LYL1) is a proto-oncogenic transcription factor found upregulated in patients with T-cell acute lymphoblastic leukemia (T-cell ALL). LYL1 has an important role in hematopoietic stem cell biology, normal hematopoiesis and leukemia. It is expressed throughout the hematopoietic lineages with the exception of T-cells. LYL1 shows two bands in the WB detection, where the upper band is the phosphorylated form and the lower band is the non-phosphorylated form (PMID: 10023675, PMID: 20844761).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for LYL1 antibody 15946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |