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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LY75/DEC-205 Polyclonal antibody (32754-1-AP)

    WB analysis using 32754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32754-1-AP (LY75/DEC-205 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LY75/DEC-205 Polyclonal antibody (32754-1-AP)

    WB analysis using 32754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32754-1-AP (LY75/DEC-205 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LY75/DEC-205 Polyclonal antibody (32754-1-AP)

    IHC staining of human tonsillitis using 32754-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32754-1-AP (LY75/DEC-205 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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LY75/DEC-205 Polyclonal antibody

LY75/DEC-205 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32754-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD 205, LY75, CD205, DEC 205, DEC-205

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDaudi cells, Raji cells, mouse thymus tissue, rat thymus tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32754-1-AP targets LY75/DEC-205 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LY75/DEC-205 fusion protein Eg3439 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 75
Calculated molecular weight198kDa
Observed molecular weight198-250 kDa
GenBank accession numberNM_002349.4
Gene Symbol CD205
Gene ID (NCBI) 4065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60449-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LY75, also known as DEC-205 or CD205, is a type I transmembrane protein belonging to the C-type lectin receptor family. It is primarily expressed on dendritic cells (DCs) and thymic epithelium, but is also found on monocytes, T and B lymphocytes, and various other tissues, including intestinal and pulmonary epithelia.

Protocols

Product Specific Protocols
WB protocol for LY75/DEC-205 antibody 32754-1-APDownload protocol
IHC protocol for LY75/DEC-205 antibody 32754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols