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  • Featured Product
  • KD/KO Validated

LUC7L2 Polyclonal antibody

LUC7L2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24202-1-AP

Synonyms

CGI 59, CGI 74, LUC7 like 2 (S. cerevisiae), LUC7B2, LUC7L2

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 24202-1-AP

    WB analysis of K-562 using 24202-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24202-1-AP (LUC7L2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24202-1-AP

    WB analysis of HeLa using 24202-1-AP

    WB result of LUC7L2 antibody (24202-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L2 transfected HeLa cells.

  • WB analysis of HeLa using 24202-1-AP

    WB analysis of HeLa using 24202-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24202-1-AP (LUC7L2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24202-1-AP

    IF Staining of HeLa using 24202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 24202-1-AP (LUC7L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

24202-1-AP targets LUC7L2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LUC7L2 fusion protein Ag21135 相同性解析による交差性が予測される生物種
Full Name LUC7-like 2 (S. cerevisiae)
Calculated molecular weight 392 aa, 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC017163
Gene symbol LUC7L2
Gene ID (NCBI) 51631
RRIDAB_2879455
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding protein LUC7L2 as a negative regulator of DNA virus-triggered innate immune response. Mechanistically, LUC7L2 directly bound to intron 3 of MITA precursor messenger RNA, inhibited its splicing and promoted its nonsense-mediated decay, leading to its downregulation at protein level (PMID:34155193). Moreover, LUC7L2 as a component of the U1 snRNP capable of reprogramming cellular metabolism through changes in alternative pre-mRNA splicing (PMID:33961773).

Protocols

Product Specific Protocols
WB protocol for LUC7L2 antibody 24202-1-APDownload protocol
IF protocol for LUC7L2 antibody 24202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols