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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LUC7L Recombinant antibody (83585-1-RR)

    WB analysis using 83585-1-RR (same clone as 83585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83585-1-RR (LUC7L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LUC7L Recombinant antibody (83585-1-RR)

    WB analysis of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    WB result of LUC7L antibody (83585-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L transfected HepG2 cells. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LUC7L Recombinant antibody (83585-1-RR)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using LUC7L Recombinant antibody (83585-1-RR)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LUC7L Recombinant antibody (83585-1-RR)

    IF Staining of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LUC7L antibody (83585-1-RR, Clone: 240311B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using LUC7L Recombinant antibody (83585-1-RR)

    FC experiment of MCF-7 using 83585-1-RR (same clone as 83585-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LUC7L Recombinant antibody (83585-1-RR, Clone:240311B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83585-1-RR

    Affinity and Kinetic Characterization of 83585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83585-1-RR against Human LUC7L were performed. The affinity constant is 99.9 pM.

  • Featured Product
  • KD/KO Validated

LUC7L Recombinant monoclonal antibody, PBS Only

LUC7L Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83585-1-PBS
Clone No.240311B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83585-1-PBS targets LUC7L in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9170

Product name: Recombinant human LUC7L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-354 aa of BC065198

Sequence: MQMGDETRQRVKFTDDRVCKSHLLDCCPHDILAGTRMDLGECTKIHDLALRADYEIASKERDLFFELDAMDHLESFIAECDRRTELAKKRLAETQEEISAEVSAKAEKVHELNEEIGKLLAKAEQLGAEGNVDESQKILMEVEKVRAKKKEAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREEREREERLSRRSGSRTRDRRRSRSRDRRRRRSRSTSRERRKLSRSRSRDRHRRHRSRSRSHSRGHRRASRDRSAKYKFSRERASREESWESGRSERGPPDWRLESSNGKMASRRSEEKEAGEI

相同性解析による交差性が予測される生物種
Full Name LUC7-like (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight38 kDa and 44 kDa
GenBank accession numberBC065198
Gene Symbol LUC7L
Gene ID (NCBI) 55692
RRIDAB_3671199
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NQ29
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa.

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