Validation Data Gallery
Tested Applications
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | mouse lung tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
28824-1-AP targets LTBP2 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29737 Product name: Recombinant human LTBP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 241-376 aa of BC078659 Sequence: ERSPNLRRSSAAGEGTLARAQPPAPQSPPAPQSPPAGTLSGLSQTHPSQQHVGLSRTVRLHPTATASSQLSSNALPPGPGLEQRDGTQQAVPLEHPSSPWGLNLTEKIKKIKIVFTPTICKQTCARGHCANSCERG 相同性解析による交差性が予測される生物種 |
| Full Name | latent transforming growth factor beta binding protein 2 |
| Calculated molecular weight | 1821 aa, 195 kDa |
| Observed molecular weight | 260 kDa |
| GenBank accession number | BC078659 |
| Gene Symbol | LTBP2 |
| Gene ID (NCBI) | 4053 |
| RRID | AB_3669670 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14767 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LTBP2, an extracellular glycoprotein mapping to chromosome 14q24, belongs to a family of latent TGF-β binding proteins (LTBPs) that regulates a well-known growth factor, TGF-β. LTBP2 is an elastic fiber-associating protein whose function in elastogenesis is not clear, as a DANCEbinding Protein. (PMID: 17581631 )It may play an integral structural role in elastic-fiber architectural organization and/or assembly. LTBP2 associates covalently with small latent TGF-beta complex via Repeat B and Repeat C. Defects in LTBP2 are the cause of primary congenital glaucoma type 3D (GLC3D). [MIM:613086] LTBP2 is a role in the structural stability of ciliary zonules, and growth and development of lens.(PMID: 20617341) LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma (PMID: 20179738 ) . LTBP2 is a biomarker of heart failure. LTBP2 has a molecular weight of 260 kDa (PMID: 20878956), and even larger molecular weights of 290 and 310 kDa were detected in tissue extracts. (PMID: 8524260)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LTBP2 antibody 28824-1-AP | Download protocol |
| IHC protocol for LTBP2 antibody 28824-1-AP | Download protocol |
| IP protocol for LTBP2 antibody 28824-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |