Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 13662-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13662-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13662-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13662-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 13662-1-AP
IP result of anti-LTA4H (IP:13662-1-AP, 4ug; Detection:13662-1-AP 1:500) with HEK-293 cells lysate 1120ug.
IHC staining of human skin using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 13662-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

LTA4H Polyclonal antibody

LTA4H Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13662-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

EC:3.3.2.6, EC:3.4.11.4, Leukotriene A-4 hydrolase, LTA 4 hydrolase, LTA4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, HeLa cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human skin tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

13662-1-AP targets LTA4H in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4568 Product name: Recombinant human LTA4H protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 260-611 aa of BC032528 Sequence: LVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVTAMLVGKDLKVD 相同性解析による交差性が予測される生物種
Full Name	leukotriene A4 hydrolase
Calculated molecular weight	611 aa, 69 kDa
Observed molecular weight	57-70 kDa
GenBank accession number	BC032528
Gene Symbol	LTA4H
Gene ID (NCBI)	4048
RRID	AB_2138747
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09960
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LTA4H(Leukotriene A-4 hydrolase), also named as LTA4, is essential for the conversion of LTA4 to LTB4, an inflammatory lipid mediator(PMID:10601658). LTA4H also possesses aminopeptidase activity that it can degrade PGP, which is a biomarker for chronic obstructive pulmonary disease and is implicated in neutrophil persistence in the lung(PMID:20813919). This protein has 4 isoforms produced by alternative splicing with the MW of 69, 59, 57, 67 kDa.

Protocols

Product Specific Protocols
WB protocol for LTA4H antibody 13662-1-AP	Download protocol
IHC protocol for LTA4H antibody 13662-1-AP	Download protocol
IP protocol for LTA4H antibody 13662-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Signal ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis Authors - Hao Cai View Article
human	WB	Proteomics Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma. Authors - Wei Gao View Article
mouse	IF	JHEP Rep Time-resolved single-cell transcriptomics reveals the landscape and dynamics of hepatic cells in sepsis-induced acute liver dysfunction Authors - Gan Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13662-1-AP

WB analysis of Jurkat using 13662-1-AP

WB analysis of HEK-293 using 13662-1-AP

WB analysis of Jurkat using 13662-1-AP

IP experiment of HEK-293 using 13662-1-AP

IHC staining of human skin using 13662-1-AP

IHC staining of human skin using 13662-1-AP

IHC staining of human brain using 13662-1-AP

IHC staining of human brain using 13662-1-AP

IHC staining of human spleen using 13662-1-AP

IHC staining of human spleen using 13662-1-AP

IHC staining of human lung using 13662-1-AP

IHC staining of human lung using 13662-1-AP

IHC staining of human ovary using 13662-1-AP

IHC staining of human ovary using 13662-1-AP

IHC staining of human heart using 13662-1-AP