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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LSS Polyclonal antibody (18693-1-AP)

    WB analysis of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18693-1-AP (LSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using LSS Polyclonal antibody (18693-1-AP)

    IP experiment of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    IP result of anti-LSS (IP:18693-1-AP, 4ug; Detection:18693-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LSS Polyclonal antibody (18693-1-AP)

    IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18693-1-AP (LSS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LSS Polyclonal antibody (18693-1-AP)

    IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18693-1-AP (LSS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LSS Polyclonal antibody (18693-1-AP)

    IF Staining of HepG2 using 18693-1-AP (same clone as 18693-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18693-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

LSS Polyclonal antibody, PBS Only

LSS Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18693-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18693-1-PBS targets LSS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6955

Product name: Recombinant human LSS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 382-732 aa of BC035638

Sequence: NGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRYVQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIFPIWALGRFSQLYPERALAGHP

相同性解析による交差性が予測される生物種
Full Name lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC035638
Gene Symbol LSS
Gene ID (NCBI) 4047
RRIDAB_2138608
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48449
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.

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