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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using LSR Polyclonal antibody (18216-1-AP)

    WB analysis of HT-29 using 18216-1-AP (same clone as 18216-1-PBS)

    HT-29 cells (LSR+) and HeLa cells (LSR-) were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using LSR Polyclonal antibody (18216-1-AP)

    WB analysis of HT-29 using 18216-1-AP (same clone as 18216-1-PBS)

    WB result of LSR antibody (18216-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LSR transfected HT-29 cells. This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LSR Polyclonal antibody (18216-1-AP)

    WB analysis using 18216-1-AP (same clone as 18216-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse liver using 18216-1-AP (same clone as 18216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using LSR Polyclonal antibody (18216-1-AP)

    IHC staining of mouse liver using 18216-1-AP (same clone as 18216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LSR Polyclonal antibody, PBS Only

LSR Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18216-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ILDR3, Lipolysis-stimulated lipoprotein receptor, LISCH, LISCH7

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18216-1-PBS targets LSR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12961

Product name: Recombinant human LSR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 339-589 aa of BC004381

Sequence: SMRVLYYMEKELANFDPSRPGPPSGRVERAMSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRRSRAYMPPRSRSRDDLYDQDDSRDFPRSRDPHYDDFRSRERPPADPRSHHHRTRDPRDNGSRSGDLPYDGRLLEEAVRKKGSEERRRPHKEEEEEAYYPPAPPPYSETDSQASRERRLKKNLALSRESLVV

相同性解析による交差性が予測される生物種
Full Name lipolysis stimulated lipoprotein receptor
Calculated molecular weight 71 kDa
Observed molecular weight 60-70 kDa, 56 kDa
GenBank accession numberBC004381
Gene Symbol LSR
Gene ID (NCBI) 51599
RRIDAB_2250137
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86X29
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).

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