Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells, HeLa cells, HepG2 cells, L02 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33966-1-AP targets LSM14A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag41250 Product name: Recombinant human LSM14A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 108-219 aa of BC016842 Sequence: MGSYGPFGRMPTYSQFSPSSLVGQQFGAVGVAGSSLTSFGTETSNSGTLPQSSAVGSAFTQDTRSLKTQLSQGRSSPQLDPLRKSPTMEQAVQTASAHLPAPAAVGRRSPVS 相同性解析による交差性が予測される生物種 |
| Full Name | LSM14A, SCD6 homolog A (S. cerevisiae) |
| Calculated molecular weight | 463 aa, 51 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC016842 |
| Gene Symbol | LSM14A |
| Gene ID (NCBI) | 26065 |
| RRID | AB_3743127 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8ND56 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LSM14A antibody 33966-1-AP | Download protocol |
| WB protocol for LSM14A antibody 33966-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |