Validation Data Gallery

Filter: AllWBIHC

WB analysis using 26951-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 26951-1-AP
WB result of Perilipin 5 antibody (26951-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.
WB analysis of MCF-7 using 26951-1-AP
WB result of Perilipin 5 antibody (26951-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.
WB analysis of pig heart using 26951-1-AP
pig heart tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 26951-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brown adipose using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).
IHC staining of mouse heart using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brown adipose using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 26951-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

Perilipin 5 Polyclonal antibody

Perilipin 5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26951-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

LSDP5, PLIN5, Perilipin-5, PAT-1, OXPAT

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, mouse liver tissue, pig heart tissue, mouse heart tissue, rat heart tissue
Positive IHC detected in	human liver tissue, mouse heart tissue, mouse brown adipose tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 24 publications below
IHC	See 2 publications below
IF	See 5 publications below
IP	See 1 publications below

Product Information

26951-1-AP targets Perilipin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25272 Product name: Recombinant human LSDP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 381-463 aa of BC131524 Sequence: ILVERPEPLPDLADLVDEVIGGPDPRWAHLDWPAQQRAWEAEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPELDF 相同性解析による交差性が予測される生物種
Full Name	lipid storage droplet protein 5
Calculated molecular weight	463 aa, 51 kDa
Observed molecular weight	51-55 kDa
GenBank accession number	BC131524
Gene Symbol	Perilipin 5
Gene ID (NCBI)	440503
RRID	AB_2880699
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q00G26
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.

Protocols

Product Specific Protocols
IHC protocol for Perilipin 5 antibody 26951-1-AP	Download protocol
WB protocol for Perilipin 5 antibody 26951-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Mol Cell Biol Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice Authors - Xinyu Bao View Article
mouse	WB	EMBO Rep miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle. Authors - Hui Wang View Article
mouse	WB	Int J Mol Sci Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression. Authors - Miljenko V Panajatovic View Article
mouse	WB	Int Immunopharmacol Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway. Authors - Kang Huo View Article
human	WB,IF	Hum Cell Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells. Authors - Hsueh-Chun Wang View Article
mouse	WB	Biochem Biophys Res Commun Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation. Authors - Jie Feng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26951-1-AP

WB analysis of MCF-7 using 26951-1-AP

WB analysis of MCF-7 using 26951-1-AP

WB analysis of pig heart using 26951-1-AP

WB analysis of mouse liver using 26951-1-AP

IHC staining of mouse brown adipose using 26951-1-AP

IHC staining of human liver using 26951-1-AP

IHC staining of human liver using 26951-1-AP

IHC staining of mouse heart using 26951-1-AP

IHC staining of mouse brown adipose using 26951-1-AP

IHC staining of mouse heart using 26951-1-AP

IHC staining of human heart using 26951-1-AP

IHC staining of human heart using 26951-1-AP

Perilipin 5 Polyclonal antibody

Perilipin 5 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice

miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle.

Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression.

Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway.

Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.

Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.