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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LRSAM1 Polyclonal antibody (24666-1-AP)

    WB analysis of Jurkat using 24666-1-AP (same clone as 24666-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LRSAM1 Polyclonal antibody (24666-1-AP)

    WB analysis of mouse liver using 24666-1-AP (same clone as 24666-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LRSAM1 Polyclonal antibody (24666-1-AP)

    WB analysis of HepG2 using 24666-1-AP (same clone as 24666-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LRSAM1 Polyclonal antibody (24666-1-AP)

    IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LRSAM1 Polyclonal antibody (24666-1-AP)

    IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRSAM1 Polyclonal antibody (24666-1-AP)

    IF Staining of A431 using 24666-1-AP (same clone as 24666-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRSAM1 antibody (24666-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LRSAM1 Polyclonal antibody, PBS Only

LRSAM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24666-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RING-type E3 ubiquitin transferase LRSAM1, RIFLE, Leucine-rich repeat and sterile alpha motif-containing protein 1, hTAL, EC:2.3.2.27

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24666-1-PBS targets LRSAM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20415

Product name: Recombinant human LRSAM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 501-723 aa of BC009239

Sequence: QEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAARIQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRIYHSS

相同性解析による交差性が予測される生物種
Full Name leucine rich repeat and sterile alpha motif containing 1
Calculated molecular weight 723 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC009239
Gene Symbol LRSAM1
Gene ID (NCBI) 90678
RRIDAB_2879665
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UWE0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LRSAM1 also named as hTAL, RIFLE orTsg101 associated ligase is a 723 amino acid protein, which contains 6 LRR repeats, 1 Ring-type zinc finger and 1 SAM domain. LRSAM1 is localizes in cytoplasm and highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. As a E3 ubiquitin protein ligase, LRSAM1 mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic and exocytic cargos, LRSAM1 gene mutation has an association with Charcot Maire Tooth disease 2P.

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