Validation Data Gallery
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Tested Applications
| Positive WB detected in | LPS treated RAW 264.7 cells, J774A.1 cells, RAW 264.7 cells, THP-1 cells, rat lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27614-1-AP targets LRRFIP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26355 Product name: Recombinant human LRRFIP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-300 aa of BC053668 Sequence: IEEQEEQMAEFYRENEEKSKELERQKHMCSVLQHKMEELKEGLRQRDELIEKHGLVIIPDGTPNGDVSHEPVAGAITVVSQEAAQVLESAGEGPLDVRLRKLAGEKEELLSQIRKLKLQLEEERQKCSRNDGTVGDLAGLQNGSDLQFIE 相同性解析による交差性が予測される生物種 |
| Full Name | leucine rich repeat (in FLII) interacting protein 2 |
| Calculated molecular weight | 400 aa, 45 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC053668 |
| Gene Symbol | LRRFIP2 |
| Gene ID (NCBI) | 9209 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y608 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LRRFIP2 (Leucine-rich repeat flightless-interacting protein 2) is a cytoplasmic protein that regulates innate immune signaling by modulating nucleic acid-sensing pathways. It interacts with flightless-I homolog and plays roles in inflammation and antiviral responses .
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LRRFIP2 antibody 27614-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |