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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRRFIP1 Recombinant antibody (84778-4-RR)

    WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LRRFIP1 Recombinant antibody (84778-4-RR)

    WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LRRFIP1 Recombinant antibody (84778-4-RR)

    WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRRFIP1 Recombinant antibody (84778-4-RR)

    IF Staining of A431 using 84778-4-RR (same clone as 84778-4-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRRFIP1 antibody (84778-4-RR, Clone: 242161C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

LRRFIP1 Recombinant monoclonal antibody, PBS Only

LRRFIP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84778-4-PBS
Clone No.242161C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242161C9, FLAP 1, FLIIAP1, GC binding factor 2, GC-binding factor 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84778-4-PBS targets LRRFIP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34760

Product name: Recombinant human LRRFIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 500-808 aa of BC108914

Sequence: EEEGEETGLRDEKPIKTEVPGSPAGTEGNCQEATGPSTVDTQNEPLDMKEPDEEKSDQQGEAFDSSQKKTKNKKKKNKKKKSPVPVETLKDVKKELTYQNTDLSEIKEEEQVKSTDRKSAVEAQNEVTENPKQKIAAESSENVDCPENPKIKLDGKLDQEGDDVQTAAEEVLADGDTLDFEDDTVQSSGPRAGGEELDEGVAKDNAKIDGATQSSPAEPKSEDADRCTLPEHESPSQDISDACEAESTERCEMSEHPSQTVRKALDSNSLENDDLSAPGREPGHFNPESREDTRGGNEKGKSKEDCTMS

相同性解析による交差性が予測される生物種
Full Name leucine rich repeat (in FLII) interacting protein 1
Observed molecular weight170 kDa
GenBank accession numberBC108914
Gene Symbol LRRFIP1
Gene ID (NCBI) 9208
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ32MZ4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

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