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Validation Data Gallery

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  • Western Blot (WB) analysis of HaCaT cells using LRRC8D Recombinant antibody (86510-1-RR)

    WB analysis of HaCaT using 86510-1-RR (same clone as 86510-1-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 86510-1-RR (LRRC8D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LRRC8D Recombinant antibody (86510-1-RR)

    WB analysis using 86510-1-RR (same clone as 86510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86510-1-RR (LRRC8D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using LRRC8D Recombinant antibody (86510-1-RR)

    IF Staining of HEK-293 using 86510-1-RR (same clone as 86510-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRRC8D antibody (86510-1-RR, Clone: 251257E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

LRRC8D Recombinant monoclonal antibody, PBS Only

LRRC8D Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86510-1-PBS
Clone No.251257E6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HsLRRC8D, Leucine-rich repeat-containing protein 5, Leucine-rich repeat-containing protein 8D, LRRC5, Volume-regulated anion channel subunit LRRC8D

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86510-1-PBS targets LRRC8D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2138

Product name: Recombinant human LRRC8D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 515-858 aa of BC024159

Sequence: LQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQLFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPLEVKEALNQDINIPFANGI

相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 8 family, member D
Calculated molecular weight 858 aa, 98 kDa
Observed molecular weight 98-120 kDa
GenBank accession numberBC024159
Gene Symbol LRRC8D
Gene ID (NCBI) 55144
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ7L1W4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.

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