Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27796-1-AP targets LRRC56 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22018 Product name: Recombinant human LRRC56 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-318 aa of BC035936 Sequence: MDLGWDRSRGPRRSTSSVRVRELSWQGLHNPCPQSKGPGSQRDRLGEQLVEEYLSPARLQALARVDDLRLVRTLEMCVDTREGSLGNFGVHLPNLDQLKLNGSHLGSLRDLGTSLGHLQVLWLARCGLADLDGIASLPALKELYASYNNISDLSPLCLLEQLEVLDLEGNSVEDLGQVRYLQLCPRLAMLTLEGNLVCLQPAPGPTNKVPRGYNYRAEVRKLIPQLQVLDEVPAAHTGPPAPPRLSQDWLAVKEAIKKGNGLPPLDCPRGAPIRRLDPELSLPETQSRASRPWPFSLLVRGGPLPEGLLSEDLAPEDN 相同性解析による交差性が予測される生物種 |
| Full Name | leucine rich repeat containing 56 |
| Calculated molecular weight | 542 aa, 59 kDa |
| GenBank accession number | BC035936 |
| Gene Symbol | LRRC56 |
| Gene ID (NCBI) | 115399 |
| RRID | AB_3669624 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYG6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Leucine-rich repeat (LRR)-containing gene 56 (LRRC56) was recently reported to be a homolog of the Outer Dynein Arm 8 gene (oda8), a gene necessary for the cytoplasmic maturation of the outer dynein arms (ODA) in the flagella of algae. LRRC56 is recruited during axoneme construction in an IFT-dependent manner and is required for the addition of dynein arms to the distal segment of the flagellum. Human LRRC56 encodes a 542 amino acid protein determined from transcriptomic studies and immunohistochemistry to be expressed in many organs, mostly in testis and pituitary gland.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LRRC56 antibody 27796-1-AP | Download protocol |
| IHC protocol for LRRC56 antibody 27796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |