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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRRC52 Polyclonal antibody (25584-1-AP)

    WB analysis using 25584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25584-1-AP (LRRC52 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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LRRC52 Polyclonal antibody

LRRC52 Polyclonal Antibody for WB, ELISA
Cat No. 25584-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LRRC52

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25584-1-AP targets LRRC52 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC52 fusion protein Ag22354 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 52
Calculated molecular weight 313 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC132981
Gene Symbol LRRC52
Gene ID (NCBI) 440699
RRIDAB_2880140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N7C0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRRC52 (leucine-rich repeat-containing protein 52) belongs to the gamma family of the BK channel accessory subunits, which potentially regulate the channel's gating properties (PMID: 22547800). It is dominantly expressed in testis and skeletal muscle, with much lower expression in several other tissues including placenta, kidney, lung, and some glands. LRRC52 has been reported as a Slo3 auxiliary subunit that helps define the specific alkalization dependence of KSper activation. LRRC52 has a calcular molecular weight of 35 kDa. This polyclonal antibody raised against 24-244aa of human LRRC52 reveals a band of 45 kDa, which is consistent with the molecular weight of glycosylated form of LRRC52 (PMID: 22084117).

Protocols

Product Specific Protocols
WB protocol for LRRC52 antibody 25584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols