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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IHC staining of mouse testis using 30156-1-AP (same clone as 30156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IHC staining of mouse testis using 30156-1-AP (same clone as 30156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IF Staining of mouse testis using 30156-1-AP (same clone as 30156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IF Staining of mouse testis using 30156-1-AP (same clone as 30156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30156-1-PBS in a different storage buffer formulation.

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LRRC50 Polyclonal antibody, PBS Only

LRRC50 Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 30156-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30156-1-PBS targets LRRC50 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC50 fusion protein Ag32822 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 50
Calculated molecular weight 725 aa, 80 kDa
GenBank accession numberBC024009
Gene Symbol LRRC50
Gene ID (NCBI) 123872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEP3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LRRC50 is a cilium-specific protein required for the stability of the ciliary architecture, it plays a role in cytoplasmic preassembly of dynein arms.