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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRRC45 Polyclonal antibody (32111-1-AP)

    WB analysis using 32111-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32111-1-AP (LRRC45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRRC45 Polyclonal antibody (32111-1-AP)

    IF Staining of A431 using 32111-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRRC45 antibody (32111-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using LRRC45 Polyclonal antibody (32111-1-AP)

    IF Staining of U-251 using 32111-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LRRC45 antibody (32111-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

LRRC45 Polyclonal antibody

LRRC45 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32111-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

LRC45, Leucine-rich repeat-containing protein 45

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, U2OS cells
Positive IF/ICC detected inA431 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32111-1-AP targets LRRC45 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36776

Product name: Recombinant human LRRC45 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 380-512 aa of BC014109

Sequence: ERKFRCQQEQLFQTRQEMTSMSAELKMRAIQAEERLDMEKRRCRQSLEDSESLRIKEVEHMTRHLEESEKAMQERVQRLEAARLSLEEELSRVKAAALSERGQAEEELIKAKSQARLEEQQRLAHLEDKLRLL

相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 45
Calculated molecular weight76 kDa
Observed molecular weight70 kDa
GenBank accession numberBC014109
Gene Symbol LRRC45
Gene ID (NCBI) 201255
RRIDAB_3670195
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96CN5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Leucine-Rich Repeat-Containing 45 (LRRC45) gene, a relatively unexplored entity in the realm of genomic studies, has recently garnered attention for its potential role in cellular processes and disease pathogenesis. Characterized by its leucine-rich repeats, a motif known for facilitating protein-protein interactions, LRRC45 is implicated in various cellular functions, including signal transduction and intracellular trafficking. LRRC45 promotes lung cancer proliferation and progression by enhancing c-MYC, Slug, MMP2, and MMP9 expression (PMID: 39326735).

Protocols

Product Specific Protocols
IF protocol for LRRC45 antibody 32111-1-APDownload protocol
WB protocol for LRRC45 antibody 32111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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