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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using LRRC32 Polyclonal antibody (26021-1-AP)

    WB analysis of HUVEC using 26021-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 26021-1-AP (LRRC32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LRRC32 Polyclonal antibody (26021-1-AP)

    IHC staining of human tonsillitis using 26021-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LRRC32 Polyclonal antibody (26021-1-AP)

    IHC staining of human tonsillitis using 26021-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using LRRC32 Polyclonal antibody (26021-1-AP)

    IHC staining of mouse spleen using 26021-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using LRRC32 Polyclonal antibody (26021-1-AP)

    IHC staining of mouse spleen using 26021-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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LRRC32 Polyclonal antibody

LRRC32 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26021-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

D11S833E, GARP, Garpin, LRRC32

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26021-1-AP targets LRRC32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC32 fusion protein Ag23423 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 32
Calculated molecular weight 72 kDa
Observed molecular weight80 kDa
GenBank accession numberBC070079
Gene Symbol LRRC32
Gene ID (NCBI) 2615
RRIDAB_2880339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14392
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats (PMID: 8180135). LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells (PMID: 27054568). It has been demonstrated as a Treg-specific activation marker (PMID: 20137067). LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor (PMID: 19651619).

Protocols

Product Specific Protocols
WB protocol for LRRC32 antibody 26021-1-APDownload protocol
IHC protocol for LRRC32 antibody 26021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

iScience

GRP94 promotes anoikis resistance and peritoneal metastasis through YAP/TEAD1 pathway in gastric cancer

Authors - Qimeng Shi
View Article