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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LRRC2 Recombinant antibody (84404-1-RR)

    IHC staining of mouse skeletal muscle using 84404-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LRRC2 Recombinant antibody (84404-1-RR)

    IHC staining of mouse skeletal muscle using 84404-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using LRRC2 Recombinant antibody (84404-1-RR)

    IF Staining of U-251 using 84404-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using LRRC2 antibody (84404-1-RR, Clone: 241132G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U-251 cells using LRRC2 Recombinant antibody (84404-1-RR)

    FC experiment of U-251 using 84404-1-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug LRRC2 Recombinant antibody (84404-1-RR, Clone:241132G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84404-1-RR

    Affinity and Kinetic Characterization of 84404-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84404-1-RR against Human LRRC2 were performed. The affinity constant is 26.2 nM.

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LRRC2 Recombinant antibody

LRRC2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84404-1-RR
Clone No.241132G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Leucine-rich repeat-containing protein 2, 241132G1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84404-1-RR targets LRRC2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LRRC2 fusion protein Ag27830 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 2
Calculated molecular weight 371 aa, 43 kDa
GenBank accession numberBC029118
Gene Symbol LRRC2
Gene ID (NCBI) 79442
RRIDAB_3671933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BYS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leucine Rich Repeat Containing 2 (LRRC2) was found to be localized to the mitochondria in human cells and transcriptionally regulated by the mitochondrial master regulator Pgc-1α. It has been reported that Lrrc2 transcript abundance correlates with that of β-MHC, a canonical marker of cardiac hypertrophy in humans, and experimentally demonstrated an elevation in Lrrc2 transcript in vitro and in vivo rodent models of cardiac hypertrophy as well as in patients with dilated cardiomyopathy. (PMID: 28158196)

Protocols

Product Specific Protocols
IHC protocol for LRRC2 antibody 84404-1-RRDownload protocol
IF protocol for LRRC2 antibody 84404-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols