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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRC2 Polyclonal antibody

LRRC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28057-1-AP

Synonyms

LRRC2

Validation Data Gallery

Applications:
  • WB analysis using 28057-1-AP

    WB analysis using 28057-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28057-1-AP (LRRC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 28057-1-AP

    IHC staining of mouse skeletal muscle using 28057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28057-1-AP (LRRC2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28057-1-AP targets LRRC2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, mouse samples.

Tested Reactivity mouse, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC2 fusion protein Ag27830 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 2
Calculated molecular weight 371 aa, 43 kDa
Observed molecular weight38 kDa
GenBank accession numberBC029118
Gene symbol LRRC2
Gene ID (NCBI) 79442
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leucine Rich Repeat Containing 2 (LRRC2) was found to be localized to the mitochondria in human cells and transcriptionally regulated by the mitochondrial master regulator Pgc-1α. It has been reported that Lrrc2 transcript abundance correlates with that of β-MHC, a canonical marker of cardiac hypertrophy in humans, and experimentally demonstrated an elevation in Lrrc2 transcript in vitro and in vivo rodent models of cardiac hypertrophy as well as in patients with dilated cardiomyopathy. (PMID: 28158196)

Protocols

Product Specific Protocols
WB protocol for LRRC2 antibody 28057-1-APDownload protocol
IHC protocol for LRRC2 antibody 28057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols