Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24434-1-AP targets LRP4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19487 Product name: Recombinant human LRP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1561-1905 aa of BC136667 Sequence: ALTQQDRWIYWTDWQTKSIQRVDKYSGRNKETVLANVEGLMDIIVVSPQRQTGTNACGVNNGGCTHLCFARASDFVCACPDEPDSQPCSLVPGLVPPAPRATGMSEKSPVLPNTPPTTLYSSTTRTRTSLEEVEGRCSERDARLGLCARSNDAVPAAPGEGLHISYAIGGLLSILLILVVIAALMLYRHKKSKFTDPGMGNLTYSNPSYRTSTQEVKIEAIPKPAMYNQLCYKKEGGPDHNYTKEKIKIVEGICLLSGDDAEWDDLKQLRSSRGGLLRDHVCMKTDTVSIQASSGSLDDTETEQLLQEEQSECSSVHTAATPERRGSLPDTGWKHERKLSSESQV 相同性解析による交差性が予測される生物種 |
| Full Name | low density lipoprotein receptor-related protein 4 |
| Calculated molecular weight | 1905 aa, 212 kDa |
| Observed molecular weight | 240 kDa |
| GenBank accession number | BC136667 |
| Gene Symbol | LRP4 |
| Gene ID (NCBI) | 4038 |
| RRID | AB_3669437 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75096 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LRP4 (low-density lipoprotein receptor-related protein 4), also known as multiple epidermal growth factor-like domains 7 (MEGF7), is a type I single transmembrane protein of the low-density lipoprotein receptor (LDLR) family. LRP4 is composed of a large extracellular region containing multiple LDLa repeats, EGF-like domains and β-propeller domains, a transmembrane region and a cytoplasmic region containing an NPxY motif and a PDZ-interacting motif (PMID: 26071838). LRP4 binds agrin and recruits it to the MuSK signaling complex, which is critical for neuromuscular junction formation (PMID: 17119023; 18957220). LPR4 mutation or malfunction is implicated in disorders including congenital myasthenic syndrome, myasthenia gravis, and diseases of bone or kidney (26071838).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LRP4 antibody 24434-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
