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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using LRP2BP Polyclonal antibody (25783-1-AP)

    WB analysis of A549 using 25783-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25783-1-AP (LRP2BP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using LRP2BP Polyclonal antibody (25783-1-AP)

    WB analysis of rat lung using 25783-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 25783-1-AP (LRP2BP Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of Jurkat cells using LRP2BP Polyclonal antibody (25783-1-AP)

    WB analysis of Jurkat using 25783-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25783-1-AP (LRP2BP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using LRP2BP Polyclonal antibody (25783-1-AP)

    IHC staining of human cerebellum using 25783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25783-1-AP (LRP2BP antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using LRP2BP Polyclonal antibody (25783-1-AP)

    IHC staining of human cerebellum using 25783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25783-1-AP (LRP2BP antibody) at dilution of 1:200 (under 40x lens).

LRP2BP Polyclonal antibody

LRP2BP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25783-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

KIAA1325, LRP2 binding protein, LRP2BP, Megalin binding protein, MegBP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, rat lung tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25783-1-AP targets LRP2BP in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22779

Product name: Recombinant human LRP2BP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 36-347 aa of BC152444

Sequence: DYTHANLVDKALQLLKERILKGDTLAYFLRGQLYFEEGWYEEALEQFEEIKEKDHQATYQLGVMYYDGLGTTLDAEKGVDYMKKILDSPCPKARHLKFAAAYNLGRAYYEGKGVKRSNEEAERLWLIAADNGNPKASVKAQSMLGLYYSTKEPKELEKAFYWHSEACGNGNLESQGALGLMYLYGQGIRQDTEAALQCLREAAERGNVYAQGNLVEYYYKMKFFTKCVAFSKRIADYDEVHDIPMIAQVTDCLPEFIGRGMAMASFYHARCLQLGLGITRDETTAKHYYSKACRLNPALADELHSLLIRQRI

相同性解析による交差性が予測される生物種
Full Name LRP2 binding protein
Calculated molecular weight 347 aa, 40 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC152444
Gene Symbol LRP2BP
Gene ID (NCBI) 55805
RRIDAB_2880238
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for LRP2BP antibody 25783-1-APDownload protocol
WB protocol for LRP2BP antibody 25783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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