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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRP2-Specific Polyclonal antibody (19700-1-AP)

    WB analysis using 19700-1-AP (same clone as 19700-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LRP2-Specific Polyclonal antibody (19700-1-AP)

    IHC staining of human kidney using 19700-1-AP (same clone as 19700-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LRP2-Specific Polyclonal antibody (19700-1-AP)

    IHC staining of human kidney using 19700-1-AP (same clone as 19700-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LRP2-Specific Polyclonal antibody (19700-1-AP)

    IHC staining of mouse kidney using 19700-1-AP (same clone as 19700-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LRP2-Specific Polyclonal antibody (19700-1-AP)

    IHC staining of mouse kidney using 19700-1-AP (same clone as 19700-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LRP2-Specific Polyclonal antibody (19700-1-AP)

    IF Staining of mouse kidney using 19700-1-AP (same clone as 19700-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LRP2-Specific Polyclonal antibody (19700-1-AP)

    IF Staining of mouse kidney using 19700-1-AP (same clone as 19700-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LRP2-Specific Polyclonal antibody (19700-1-AP)

    IF Staining of mouse kidney using 19700-1-AP (same clone as 19700-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19700-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LRP2-Specific Polyclonal antibody, PBS Only

LRP2-Specific Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 19700-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

LRP2, DBS, Fx1a, Glycoprotein 330, gp330

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19700-1-PBS targets LRP2-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name low density lipoprotein-related protein 2
Calculated molecular weight 522 kDa
Observed molecular weight522 kDa
GenBank accession numberNM_004525
Gene Symbol LRP2
Gene ID (NCBI) 4036
RRIDAB_10640428
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP98164
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS).

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