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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Raji using 19498-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Jurkat using 19498-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    IF Staining of Jurkat using 19498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LRMP antibody (19498-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    FC experiment of Jurkat using 19498-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug LRMP Polyclonal antibody (19498-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

LRMP Polyclonal antibody

LRMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19498-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Inositol 1,4,5-triphosphate receptor associated 2, IRAG2, JAW1, Lymphoid-restricted membrane protein, Processed inositol 1,4,5-triphosphate receptor associated 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, Jurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19498-1-AP targets LRMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name lymphoid-restricted membrane protein
Calculated molecular weight 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_006152
Gene Symbol LRMP
Gene ID (NCBI) 4033
RRIDAB_10640430
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12912
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRMP, also named as JAW1, is a lymphoid restricted integral membrane protein that is localised to the cytoplasmic face of the endoplasmic reticulum.

Protocols

Product Specific Protocols
IF protocol for LRMP antibody 19498-1-APDownload protocol
IHC protocol for LRMP antibody 19498-1-APDownload protocol
WB protocol for LRMP antibody 19498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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