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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRDD Polyclonal antibody

LRDD Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12119-1-AP

Synonyms

LRDD, PIDD, PIDD1

Validation Data Gallery

Applications:
  • WB analysis of L02 using 12119-1-AP

    WB analysis of L02 using 12119-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12119-1-AP

    WB analysis of HEK-293 using 12119-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 12119-1-AP

    WB analysis of L02 using 12119-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 12119-1-AP

    IP experiment of L02 using 12119-1-AP

    IP result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.

  • IHC staining of human pancreas using 12119-1-AP

    IHC staining of human pancreas using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human lung using 12119-1-AP

    IHC staining of human lung using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human lung using 12119-1-AP

    IHC staining of human lung using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human lung cancer using 12119-1-AP

    IHC staining of human lung cancer using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human lung cancer using 12119-1-AP

    IHC staining of human lung cancer using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human liver using 12119-1-AP

    IHC staining of human liver using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human liver using 12119-1-AP

    IHC staining of human liver using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human pancreas using 12119-1-AP

    IHC staining of human pancreas using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IF Staining of HeLa using 12119-1-AP

    IF Staining of HeLa using 12119-1-AP

    Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inL02 cells, HEK-293 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman lung tissue, human pancreas tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy PIDD1 Ready-To-Use IHC Kit. PIDD1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12119-1-AP targets LRDD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRDD fusion protein Ag2761 相同性解析による交差性が予測される生物種
Full Name leucine-rich repeats and death domain containing
Calculated molecular weight 893 aa, 98 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC014904
Gene symbol LRDD
Gene ID (NCBI) 55367
RRIDAB_10694424
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

P53-induced protein with a death domain (PIDD/LRDD) is a component of the PIDDosome. PIDD contains 910 residues with 7 leucine rich repeats (LRRs), 2 ZU-5 domains and a C-terminal death domain (DD). PIDD can be cleaved into shorter fragments generating a PIDD-N fragment of 48 kD (residues 1-446), a PIDD-C fragment of 51 kD (residues 447-910) and a PIDD-CC fragment of 37 kD (residues 589-910). Auto-cleavage of PIDD determines the downstream signaling events. The PIDD-C fragment mediates activation of NFκB via the recruitment of RIP1 and NEMO, while PIDD-CC causes caspase-2 activation, which leads to apoptosis.

Protocols

Product Specific Protocols
WB protocol for LRDD antibody 12119-1-APDownload protocol
IHC protocol for LRDD antibody 12119-1-APDownload protocol
IF protocol for LRDD antibody 12119-1-APDownload protocol
IP protocol for LRDD antibody 12119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neural Regen Res

Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation.

Authors - Wei-Na Chai
View Article