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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LRCH1 Polyclonal antibody (25017-1-AP)

    WB analysis of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25017-1-AP (LRCH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using LRCH1 Polyclonal antibody (25017-1-AP)

    IP experiment of HeLa using 25017-1-AP (same clone as 25017-1-PBS)

    IP result of anti-LRCH1 (IP:25017-1-AP, 4ug; Detection:25017-1-AP 1:300) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using LRCH1 Polyclonal antibody (25017-1-AP)

    IHC staining of mouse cerebellum using 25017-1-AP (same clone as 25017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using LRCH1 Polyclonal antibody (25017-1-AP)

    IHC staining of mouse cerebellum using 25017-1-AP (same clone as 25017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LRCH1 Polyclonal antibody (25017-1-AP)

    IF Staining of MCF-7 using 25017-1-AP (same clone as 25017-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using LRCH1 antibody (25017-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25017-1-PBS in a different storage buffer formulation.

LRCH1 Polyclonal antibody, PBS Only

LRCH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25017-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Neuronal protein 81, Leucine-rich repeat and calponin homology domain-containing protein 1, KIAA1016, CHDC1, Calponin homology domain-containing protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25017-1-PBS targets LRCH1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18728

Product name: Recombinant human LRCH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-549 aa of BC112937

Sequence: NIMEEEQIIKEDSCHRLSPVKGEFHQEFQPEPSLLGDSTNSGEERDQFTDRADGLHSEFMNYKARAEDCEELLRIEEDVHWQTEGIISSSKDQDMDIAMIEQLREAVDLLQDPNGLSTDITERSVLNLYPMGSAEALELQDSALNGQIQLETSPVCEVQSDLTLQSNGSQYSPNEIRENSPAVSPTTNSTAPFGLKP

相同性解析による交差性が予測される生物種
Full Name leucine-rich repeats and calponin homology (CH) domain containing 1
Calculated molecular weight 728 aa, 80 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC112937
Gene Symbol LRCH1
Gene ID (NCBI) 23143
RRIDAB_2879848
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2L9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.

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