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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPP Polyclonal antibody (25045-1-AP)

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LPP Polyclonal antibody (25045-1-AP)

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using LPP Polyclonal antibody (25045-1-AP)

    WB analysis of MCF-7 using 25045-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LPP Polyclonal antibody (25045-1-AP)

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LPP Polyclonal antibody (25045-1-AP)

    IP experiment of HeLa using 25045-1-AP

    IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human kidney using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human kidney using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LPP Polyclonal antibody (25045-1-AP)

    IF Staining of HeLa using 25045-1-AP

    Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

LPP Polyclonal antibody

LPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25045-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon, mouse heart, rat heart
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25045-1-AP targets LPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19205

Product name: Recombinant human LPP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC130584

Sequence: MSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPPPPPPLDDSSALPSISGNFPPPPPLDEEAFKVQGNPGGKTLEERRSSLDAEIDSLTSILADLECSSPYKPRPPQSSTGSTASPPVSTPVTGHKRMVIPNQPPLTATKKSTLKPQPAPQAGPIPVAPIGTLKPQPQPVPASYTTASTSSRPTFNVQVKSAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQCPPPSTRGGMDYAYIPPPGLQPEPGYGYAPNQGRYYEGYYAAGPGYGGRNDSDPTYGQQGHPNTWKREPGYTPPGAGNQNPPGMYPVTG

相同性解析による交差性が予測される生物種
Full Name LIM domain containing preferred translocation partner in lipoma
Calculated molecular weight 612 aa, 66 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC130584
Gene Symbol LPP
Gene ID (NCBI) 4026
RRIDAB_2879869
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93052
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).

Protocols

Product Specific Protocols
WB protocol for LPP antibody 25045-1-APDownload protocol
IHC protocol for LPP antibody 25045-1-APDownload protocol
IF protocol for LPP antibody 25045-1-APDownload protocol
IP protocol for LPP antibody 25045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer

Authors - Kanghua Xiao
View Article