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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of mouse brain using 20045-1-AP (same clone as 20045-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of mouse colon using 20045-1-AP (same clone as 20045-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of HepG2 using 20045-1-AP (same clone as 20045-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of COLO 320 using 20045-1-AP (same clone as 20045-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of human liver using 20045-1-AP (same clone as 20045-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of rat brain using 20045-1-AP (same clone as 20045-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    IP experiment of mouse brain using 20045-1-AP (same clone as 20045-1-PBS)

    IP result of anti-LPHN3 (IP:20045-1-AP, 4ug; Detection:20045-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    IHC staining of mouse cerebellum using 20045-1-AP (same clone as 20045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20045-1-AP (LPHN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20045-1-PBS in a different storage buffer formulation.

LPHN3 Polyclonal antibody, PBS Only

LPHN3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20045-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ADGRL3, Adhesion G protein-coupled receptor L3, Calcium-independent alpha-latrotoxin receptor 3, CIRL 3, CIRL3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20045-1-PBS targets LPHN3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name latrophilin 3
Calculated molecular weight 162 kDa
Observed molecular weight 139 kDa
GenBank accession numberNM_015236
Gene Symbol LPHN3
Gene ID (NCBI) 23284
RRIDAB_10665552
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAR2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.

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