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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using LPHN1 Polyclonal antibody (30142-1-AP)

    IHC staining of mouse brain using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LPHN1 Polyclonal antibody (30142-1-AP)

    IHC staining of mouse brain using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using LPHN1 Polyclonal antibody (30142-1-AP)

    IHC staining of rat kidney using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using LPHN1 Polyclonal antibody (30142-1-AP)

    IHC staining of rat kidney using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LPHN1 Polyclonal antibody (30142-1-AP)

    IHC staining of mouse kidney using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LPHN1 Polyclonal antibody (30142-1-AP)

    IF Staining of mouse kidney using 30142-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LPHN1 Polyclonal antibody (30142-1-AP)

    IF Staining of mouse kidney using 30142-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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LPHN1 Polyclonal antibody

LPHN1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30142-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

CIRL-1, CIRL1, CIRL 1, Calcium-independent alpha-latrotoxin receptor 1, Adhesion G protein-coupled receptor L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue, rat kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30142-1-AP targets LPHN1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPHN1 fusion protein Ag31093 相同性解析による交差性が予測される生物種
Full Name latrophilin 1
Calculated molecular weight 163 kDa
GenBank accession numberNM_001008701
Gene Symbol LPHN1
Gene ID (NCBI) 22859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Latrophilin-1 (LPHN1) was isolated as the main high-affinity receptor for α-latrotoxin from black widow spider venom, a powerful presynaptic secretagogue (PMID: 30967757). aGPCR latrophilin 1 (ADGRL1/LPHN1) is involved in controlling energy balance and food intake. Although this receptor is present in adipocytes and many neurons, where it is essential for axon growth cone formation and neuronal. It is present in Adipocytes and many neurons, where it is essential for Axon growth cone formation and neuronal function (PMID: 38664368).

Protocols

Product Specific Protocols
IHC protocol for LPHN1 antibody 30142-1-APDownload protocol
IF protocol for LPHN1 antibody 30142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols