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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPAR4 Polyclonal antibody (22165-1-AP)

    WB analysis using 22165-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LPAR4 Polyclonal antibody (22165-1-AP)

    WB analysis of HL-60 using 22165-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LPAR4 Polyclonal antibody (22165-1-AP)

    WB analysis of mouse brain using 22165-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using LPAR4 Polyclonal antibody (22165-1-AP)

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LPAR4 Polyclonal antibody (22165-1-AP)

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SW 1990 cells using LPAR4 Polyclonal antibody (22165-1-AP)

    IF Staining of SW 1990 using 22165-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SW 1990 cells using LPAR4 antibody (22165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

LPAR4 Polyclonal antibody

LPAR4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22165-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LPA4, LPA receptor 4, LPA 4, G-protein coupled receptor 23, GPR23

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells, mouse brain tissue, HL-60 cells, 3T3-L1 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSW 1990 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

22165-1-AP targets LPAR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17817

Product name: Recombinant human LPAR4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 311-370 aa of BC069996

Sequence: YYFTLESFQKSFYINAHIRMESLFKTETPLTTKPSLPAIQEEVSDQTTNNGGELMLESTF

相同性解析による交差性が予測される生物種
Full Name lysophosphatidic acid receptor 4
Calculated molecular weight 370 aa, 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC069996
Gene Symbol LPAR4
Gene ID (NCBI) 2846
RRIDAB_11182491
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99677
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for LPAR4 antibody 22165-1-APDownload protocol
IHC protocol for LPAR4 antibody 22165-1-APDownload protocol
WB protocol for LPAR4 antibody 22165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Inhibitory effects of β-galactoside α2,6-sialyltransferase 1 on the Hippo pathway in breast cancer cells

Authors - Qinglei Hang
View Article
humanWB

Nat Cell Biol

Pancreatic cancer cells upregulate LPAR4 in response to isolation stress to promote an ECM-enriched niche and support tumour initiation

Authors - Chengsheng Wu
View Article
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