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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LOXL1 Recombinant monoclonal antibody (86534-2-RR)

    WB analysis using 86534-2-RR (same clone as 86534-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86534-2-RR (LOXL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86534-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using LOXL1 Recombinant monoclonal antibody (86534-2-RR)

    IF Staining of U-87 MG using 86534-2-RR (same clone as 86534-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using LOXL1 antibody (86534-2-RR, Clone: 251345E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86534-2-PBS in a different storage buffer formulation.

LOXL1 Recombinant monoclonal antibody, PBS Only

LOXL1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86534-2-PBS
Clone No.251345E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:1.4.3.13, LOL, LOXL, LOXL 1, LOXL-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86534-2-PBS targets LOXL1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24293

Product name: Recombinant human LOXL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 283-366 aa of BC015090

Sequence: TPGFEQAYPDPGPEAAQAHGGDPRLGWYPPYANPPPEAYGPPRALEPPYLPVRSSDTPPPGGERNGAQQGRLSVGSVYRPNQNG

相同性解析による交差性が予測される生物種
Full Name lysyl oxidase-like 1
Calculated molecular weight 63 kDa
Observed molecular weight60-63 kDa
GenBank accession numberBC015090
Gene Symbol LOXL1
Gene ID (NCBI) 4016
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ08397
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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