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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis using 66043-1-Ig (same clone as 66043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    WB result of LONP1 antibody (66043-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-LONP1 transfected Jurkat cells. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of RAW 264.7 using 66043-1-Ig (same clone as 66043-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of HSC-T6 using 66043-1-Ig (same clone as 66043-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of HEK-293 using 66043-1-Ig (same clone as 66043-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of L02 using 66043-1-Ig (same clone as 66043-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of C6 cells using 66043-1-Ig (LONP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using LONP1 antibody (66043-1-Ig, Clone: 1C6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50672-1

    Cytometric bead array standard curve of MP50672-1

    Cytometric bead array standard curve of MP50672-1, LONP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66043-1-PBS. Detection antibody: 66043-2-PBS. Standard:Ag7306. Range: 1.563-100 ng/mL.

  • Featured Product
  • KD/KO Validated

LONP1 Monoclonal antibody, PBS Only (Capture)

LONP1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66043-1-PBS
Clone No.1C6C12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66043-1-PBS targets LONP1 as part of a matched antibody pair:

MP50672-1: 66043-1-PBS capture and 66043-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7306

Product name: Recombinant human LONP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 614-959 aa of BC000235

Sequence: EQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER

相同性解析による交差性が予測される生物種
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2881494
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP36776
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

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