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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LMTK3 Polyclonal antibody (22899-1-AP)

    WB analysis using 22899-1-AP (same clone as 22899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22899-1-AP (LMTK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LMTK3 Polyclonal antibody (22899-1-AP)

    IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LMTK3 Polyclonal antibody (22899-1-AP)

    IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LMTK3 Polyclonal antibody (22899-1-AP)

    IHC staining of human lung cancer using 22899-1-AP (same clone as 22899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

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LMTK3 Polyclonal antibody, PBS Only

LMTK3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22899-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22899-1-PBS targets LMTK3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lemur tyrosine kinase 3
Calculated molecular weight 154 kDa
Observed molecular weight~155 kDa
GenBank accession numberNM_001080434
Gene Symbol LMTK3
Gene ID (NCBI) 114783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96Q04
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LMTK3 is enriched at a perinuclear structure around Golgi membranes, it is present as a dotted pattern throughout neurites, and localized at neuronal synapses. It is also expected to be expressed in brain and duodenum. LMTK3 is a serine/threonine protein kinase expressed predominantly in brain. LMTK3 regulated ER-alpha stability and activity directly at the mRNA level via downregulation of PKC catalytic activity, resulting in less phosphorylated AKT at ser473. In vitro kinase assays indicated that LMTK3 inhibited the ability of PKC to phosphorylate ER-alpha, thereby affecting ER-alpha activity indirectly at the protein level by protecting it from proteasomal degradation. In agreement, analysis with a breast tumor xenograft mouse model revealed that knockdown of LMTK3 reduced proliferation of ER-alpha-positive breast cancer cells and reduced tumor growth. In addition, LMTK3 abundance and intronic polymorphisms were significantly associated with human cancers, overall patient survival, and predicted response to endocrine therapies. The calculated molecular weight of LMTK3 is 153 kDa.