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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LMO7 Polyclonal antibody (29392-1-AP)

    WB analysis using 29392-1-AP (same clone as 29392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29392-1-AP (LMO7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29392-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LMO7 Polyclonal antibody (29392-1-AP)

    WB analysis of HeLa using 29392-1-AP (same clone as 29392-1-PBS)

    WB result of LMO7 antibody (29392-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LMO7 transfected HeLa cells. This data was developed using the same antibody clone with 29392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using LMO7 Polyclonal antibody (29392-1-AP)

    IHC staining of mouse heart using 29392-1-AP (same clone as 29392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using LMO7 Polyclonal antibody (29392-1-AP)

    IHC staining of mouse heart using 29392-1-AP (same clone as 29392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LMO7 Polyclonal antibody (29392-1-AP)

    IHC staining of mouse skeletal muscle using 29392-1-AP (same clone as 29392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29392-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LMO7 Polyclonal antibody (29392-1-AP)

    IHC staining of mouse skeletal muscle using 29392-1-AP (same clone as 29392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29392-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LMO7 Polyclonal antibody (29392-1-AP)

    IF Staining of MCF-7 using 29392-1-AP (same clone as 29392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMO7 antibody (29392-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29392-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LMO7 Polyclonal antibody, PBS Only

LMO7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29392-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FBX20, LOMP

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29392-1-PBS targets LMO7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30016

Product name: Recombinant human LMO7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 695-838 aa of NM_005358

Sequence: KIYGENGSKSMSDVSAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEEREEIEKQALEKSKRSSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEGKRPPTMTVSEASYQSERVEEKGATY

相同性解析による交差性が予測される生物種
Full Name LIM domain 7
Calculated molecular weight 193 kDa
Observed molecular weight 140-160 kDa
GenBank accession numberNM_005358
Gene Symbol LMO7
Gene ID (NCBI) 4008
RRIDAB_3086128
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWI1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).

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