Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, Raji cells, mouse brain tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lung cancer tissue, human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells, SH-SY5Y cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 32 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
10895-1-AP targets Lamin B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1335 Product name: Recombinant human LMNB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-600 aa of BC006551 Sequence: EELRSQHDEQVRLYKLELEQTYQAKLDSAKLSSDQNDKAASAAREELKEARMRLESLSYQLSGLQKQASAAEDRIRELEEAMAGERDKFRKMLDAKEQEMTEMRDVMQQQLAEYQELLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSGSLSATGRLGRSKRKRLEVEEPLGSGPSVLGTGTGGSGGFHLAQQASASGSVSIEEIDLEGKFVQLKNNSDKDQSLGNWRIKRQVLEGEEIAYKFTPKYILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM 相同性解析による交差性が予測される生物種 |
| Full Name | lamin B2 |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 66-68 kDa |
| GenBank accession number | BC006551 |
| Gene Symbol | Lamin B2 |
| Gene ID (NCBI) | 84823 |
| RRID | AB_2136411 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| IHC protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| IP protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| WB protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion
| ||
Mol Cell MLKL activates the cGAS-STING pathway by releasing mitochondrial DNA upon necroptosis induction | ||
Nat Chem Biol Fructose-1,6-bisphosphatase 1 dephosphorylates and inhibits TERT for tumor suppression | ||
Sci Adv 53BP1 orchestrates sequence feature of RAG targets to balance DNA repair outcomes during V(D)J recombination. | ||
Pharmacol Res YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the Mediator complex in colorectal cancer | ||
Phytomedicine Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts |