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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LMF2 Polyclonal antibody (16249-1-AP)

    WB analysis of HeLa using 16249-1-AP (same clone as 16249-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16249-1-AP (LMF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16249-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using LMF2 Polyclonal antibody (16249-1-AP)

    WB analysis of human brain using 16249-1-AP (same clone as 16249-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16249-1-AP (LMF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16249-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LMF2 Polyclonal antibody (16249-1-AP)

    IHC staining of human kidney using 16249-1-AP (same clone as 16249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16249-1-AP (LMF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16249-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LMF2 Polyclonal antibody (16249-1-AP)

    IHC staining of human kidney using 16249-1-AP (same clone as 16249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16249-1-AP (LMF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16249-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LMF2 Polyclonal antibody (16249-1-AP)

    IF Staining of MCF-7 using 16249-1-AP (same clone as 16249-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using 16249-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16249-1-PBS in a different storage buffer formulation.

LMF2 Polyclonal antibody, PBS Only

LMF2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16249-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LMF 2, TMEM112B, TMEM153

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16249-1-PBS targets LMF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9208

Product name: Recombinant human LMF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 394-610 aa of BC014652

Sequence: VPYSYVEPGTHGRLWTGAHRLFGAVEHLQLANSYGLFRRMTGLGGRPEVVLEGSYDGHHWTEIEFMYKPGNLSRPPPVVVPHQPRLDWQMWFAALGPHTHSPWFTSLVLRLLQGKEPVIRLVQSQVARYPFHKQPPTYVRAQRYKYWFSQPGEQGQWWRRQWVEEFFPSVSLGDPTLETLLRQFGLQEKSPPRTRSANSTLAQALHWTRSQLSPLEA

相同性解析による交差性が予測される生物種
Full Name lipase maturation factor 2
Calculated molecular weight 682 aa, 77 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC014652
Gene Symbol LMF2
Gene ID (NCBI) 91289
RRIDAB_2136142
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU23
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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