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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of mouse testis using 12154-1-AP (same clone as 12154-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of rat testis using 12154-1-AP (same clone as 12154-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of mouse liver using 12154-1-AP (same clone as 12154-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of HepG2 using 12154-1-AP (same clone as 12154-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of A549 using 12154-1-AP (same clone as 12154-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12154-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using LMBR1L Polyclonal antibody (12154-1-AP)

    IP experiment of HepG2 using 12154-1-AP (same clone as 12154-1-PBS)

    IP result of anti-LMBR1L (IP:12154-1-AP, 4ug; Detection:12154-1-AP 1:500) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 12154-1-PBS in a different storage buffer formulation.

LMBR1L Polyclonal antibody, PBS Only

LMBR1L Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12154-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12154-1-PBS targets LMBR1L in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2787

Product name: Recombinant human LMBR1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 219-490 aa of BC015015

Sequence: RMFSVTGKLLVKPRLLEDLEEQLYCSAFEEAALTRRICNPTSCWLPLDMELLHRQVLALQTQRVLLEKRRKASAWQRNLGYPLAMLCLLVLTGLSVLIVAIHILELLIDEAAMPRGMQGTSLGQVSFSKLGSFGAVIQVVLIFYLMVSSVVGFYSSPLFRSLRPRWHDTAMTQIIGNCVCLLVLSSALPVFSRTLGLTRFDLLGDFGRFNWLGNFYIVFLYNAAFAGLTTLCLVKTFTAAVRAELIRAFGLDRLPLPVSGFPQASRKTQHQ

相同性解析による交差性が予測される生物種
Full Name limb region 1 homolog (mouse)-like
Calculated molecular weight 489 aa, 55 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC015015
Gene Symbol LMBR1L
Gene ID (NCBI) 55716
RRIDAB_2136130
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX01
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LMBR1L, also known as LIMR (lipocalin-1-interacting membrane receptor), interacts with lipocalin-1 (Lcn-1), a lipocalin member produced by a number of secretoric glands and tissues and known to bind a variety of lipophilic compounds. LMBR1L is a 489-amino acid protein with a predicted molecular mass of 55 kD and 9 putative transmembrane helices. LMBR1L has been shown to mediate cellular uptake of LMBR1L, thus it is characterized as a endocytic receptor (PMID: 11287427; 12591932). Alternatively spliced transcript variants encoding different isoforms have been described.

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