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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LLGL2 Polyclonal antibody

LLGL2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15993-1-AP

Synonyms

HGL, LGL2, LLGL2

Validation Data Gallery

Applications:
  • WB analysis using 15993-1-AP

    WB analysis using 15993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15993-1-AP (LLGL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15993-1-AP

    IF Staining of HepG2 using 15993-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LLGL2 antibody (15993-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

15993-1-AP targets LLGL2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LLGL2 fusion protein Ag8867 相同性解析による交差性が予測される生物種
Full Name lethal giant larvae homolog 2 (Drosophila)
Calculated molecular weight356aa,40 kDa; 1020aa,113 kDa
Observed molecular weight120 kDa
GenBank accession numberBC010879
Gene symbol LLGL2
Gene ID (NCBI) 3993
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LLGL2 antibody 15993-1-APDownload protocol
IF protocol for LLGL2 antibody 15993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols