Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, SH-SY5Y cells, K-562 cells, HL-60 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86712-3-RR targets LIX1L in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag22209 Product name: Recombinant human LIX1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-102 aa of BC035727 Sequence: METMRAQRLQPGVGTSGRGTLRALRPGVTGAAAATATPPAGPPPAPPPPAPPPPPLLLSGAPGLPLPPGAAGSPAVLREAVEAVVRSFAKHTQGYGRVNVVE 相同性解析による交差性が予測される生物種 |
| Full Name | Lix1 homolog (mouse)-like |
| Calculated molecular weight | 337 aa, 37 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC035727 |
| Gene Symbol | LIX1L |
| Gene ID (NCBI) | 128077 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IVB5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Limb expression 1-like protein (LIX1L) is a putative RNA binding protein that contains a double-stranded RNA-binding motif. LIX1L is highly expressed in various cancer tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for LIX1L antibody 86712-3-RR | Download protocol |
| WB protocol for LIX1L antibody 86712-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |