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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis of HepG2 using 21133-1-AP (same clone as 21133-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis using 21133-1-AP (same clone as 21133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis of mouse liver using 21133-1-AP (same clone as 21133-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis of mouse liver using 21133-1-AP (same clone as 21133-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIPC Polyclonal antibody (21133-1-AP)

    IHC staining of human lung cancer using 21133-1-AP (same clone as 21133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21133-1-AP (LIPC Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIPC Polyclonal antibody (21133-1-AP)

    IHC staining of human lung cancer using 21133-1-AP (same clone as 21133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21133-1-AP (LIPC Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LIPC Polyclonal antibody (21133-1-AP)

    IF Staining of HepG2 using 21133-1-AP (same clone as 21133-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 21133-1-AP (LIPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LIPC Polyclonal antibody, PBS Only

LIPC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21133-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21133-1-PBS targets LIPC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15538

Product name: Recombinant human LIPC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 213-463 aa of BC146659

Sequence: DASFVDAIHTFTREHMGLSVGIKQPIGHYDFYPNGGSFQPGCHFLELYRHIAQHGFNAITQTIKCSHERSVHLFIDSLLHAGTQSMAYPCGDMNSFSQGLCLSCKKGRCNTLGYHVRQEPRSKSKRLFLVTRAQSPFKVYHYQLKIQFINQTETPIQTTFTMSLLGTKEKMQKIPITLGKGIASNKTYSFLITLDVDIGELIMIKFKWENSAVWANVWDTVQTIIPWSTGPRHSGLVLKTIRVKAGETQQR

相同性解析による交差性が予測される生物種
Full Name lipase, hepatic
Calculated molecular weight 499 aa, 56 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC146659
Gene Symbol LIPC
Gene ID (NCBI) 3990
RRIDAB_11041715
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11150
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LIPC, also named as HTGL and HL, belongs to the AB hydrolase superfamily and Lipase family. It has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. LIPC is an important enzyme in HDL metabolism. It binds heparin.

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